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. 2022 Mar 22;11:e74650. doi: 10.7554/eLife.74650

Appendix 1—key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Genetic reagent (Danio rerio) Tg[hsp:gal4]kca4 PMID: 11850174 ZDB-ALT-020918-6 Reugels/Campos-Ortega lab (Köln University)
Genetic reagent (Danio rerio) Tg[UAS:EGFP] PMID: 11336499 N/A
Genetic reagent (Danio rerio) Tg[UAS:MYFP] PMID: 1702063 N/A
Genetic reagent (Danio rerio) Tg[ath5:GFP] rw021 PMID: 12702661 N/A
Genetic reagent (Danio rerio) Tg[ptf1a:mCherry-CAAX]oki067 This paper N/A See ‘Materials and methods’
Genetic reagent (Danio rerio) Tg[Gal4-VP16,UAS:EGFP]xfz43 or xfz43 PMID: 19712466 ZDB-ALT-100201-1 ZIRC
Genetic reagent (Danio rerio) Tg[Gal4-VP16,UAS:EGFP]xfz3 or xfz3 PMID: 19712466 ZDB-ALT-100201-2 ZIRC
Genetic reagent (Danio rerio) Tg[hs:mCherry-tagged Bcl2]oki029 PMID: 33060680 ZDB-ALT-210524-5
Genetic reagent (Danio rerio) Tg[hsp:WT.Strip1-GFP] oki068 This paper N/A See ‘Materials and methods’
Genetic reagent (Danio rerio) Tg[hsp:Mut.Strip1-GFP] oki069 This paper N/A See ‘Materials and methods’
Genetic reagent (Danio rerio) strip1rw147 This paper N/A See ‘Materials and methods’
Genetic reagent (Danio rerio) strip1crisprΔ10 or strip1oki8 This paper N/A See ‘Materials and methods’
Genetic reagent (Danio rerio) roy PMID: 28760346 ZDB-GENE-040426-1168
Antibody anti-acetylated α-tubulin (mouse monoclonal) Sigma-Aldrich T6793 IF: 1:1000
Antibody anti-Pax6 (rabbit polyclonal) BioLegned 901,301 IF: 1:500
Antibody anti-Prox1 (rabbit polyclonal) Genetex GTX128354 IF: 1:500
Antibody anti-PCNA (mouse monoclonal) Sigma-Aldrich P8825 IF: 1:200
Antibody Zpr1 (mouse monoclonal) ZIRC ZDB-ATB-081002-43 IF: 1:100
Antibody Zpr3 (mouse monoclonal) ZIRC ZDB-ATB-081002-45 IF: 1:100
Antibody anti-glutamine synthetase (mouse monoclonal) Sigma-Aldrich MAB302 IF: 1:150
Antibody Zn5 (mouse monoclonal) ZIRC ZDB-ATB-081002-19 IF: 1:50
Antibody anti-parvalbumin (mouse monoclonal) Merck Millipore MAB1572 IF: 1:500
Antibody anti-p-Jun (rabbit polyclonal) Cell Signaling 9164S IF: 1:100
Antibody anti-Strip1 (rabbit polyclonal) This paper N/A See ‘Materials and methods’IF: 1:1000WB: 1:500
Antibody anti-rabbit Alexa 488 secondary antibody (goat polyclonal) Life Technologies A11034 IF: 1:500
Antibody anti-mouse Alexa 488 secondary antibody (goat polyclonal) Life Technologies A11029 IF: 1:500
Antibody anti-mouse Alexa 546 secondary antibody (goat polyclonal) Life Technologies A11030 IF: 1:500
Antibody anti-mouse Alexa 647 secondary antibody (goat polyclonal) Life Technologies A21236 IF: 1:500
Antibody anti-rabbit IgG, HRP-linked Antibody (goat polyclonal) Cell Signaling 7074 WB: 1:5000
Antibody anti-GFP (rabbit polyclonal) Thermo Fisher Scientific A11122 WB: 1:500
Antibody anti-Strn3 (rabbit polyclonal) Thermo Fisher Scientific PA5-31368 WB: 1:1000
Antibody anti β-actin (mouse monoclonal) Merck Millipore A5441 WB: 1:5000
Antibody anti β-actin (rabbit polyclonal) Abcam AB8227 WB: 1:5000
Recombinant DNA reagent pTol2[ptf1a:mCherry-CAAX](plasmid) This paper N/A See ‘Materials and methods’
Recombinant DNA reagent pG1[ptf1a:GFP](plasmid) PMID: 23035102 N/A Francesco Argenton Laboratory (University of Padova)
Recombinant DNA reagent pTol2[hsp:WT.Strip1-GFP](plasmid) This paper N/A See ‘Materials and methods’
Recombinant DNA reagent pTol2[hsp:Mut.Strip1-GFP](plasmid) This paper N/A See ‘Materials and methods’
Recombinant DNA reagent pBluescript SK (+)(plasmid) Stratagene N/A
Recombinant DNA reagent pT2AL200R150G(plasmid) PMID: 16959904 N/A Dr. Koichi Kawakami (Institute of Genetics)
Recombinant DNA reagent pB[ath5:Gal4-VP16](plasmid) This paper N/A See ‘Materials and methods’
Recombinant DNA reagent pZNYX-Gal4VP16(plasmid) PMID: 17020638 N/A Rachel Wong Laboratory(University of Washington)
Sequence-based reagent Standard control MO (STD-MO) GeneTools N/A 5′-CCTCTTACCTCAGTTACAATTTATA-3′Same concentration for each MO experiment
Sequence-based reagent Strip1 morpholino (MO-strip1) GeneTools N/A 5′- TAGCACATAAACCGACACCGTCCAT-3′250 μM
Sequence-based reagent Ath5 morpholino (MO-ath5) GeneTools ZDB-MRPHLNO-100405-2 5′-TTCATGGCTCTTCAAAAAAGTCTCC-3′250 μM
Sequence-based reagent Striatin3 morpholino (MO-strn3) GeneTools N/A 5′- CCTGCTAGAAGTCGCCGATTGTTAC -3′250 μM
Sequence-based reagent Jun morpholino (MO-jun) GeneTools ZDB-MRPHLNO-080908-1 5′- CTTGGTAGACATAGAAGGCAAAGCG -3′125 μM
Peptide, recombinant protein Cas9 protein FASMAC GE-006-S
Commercial assay or kit JB-4 Embedding Kit Polysciences 00226-1
Commercial assay or kit In Situ Cell Death Detection Kit, TMR Red Roche 12156792910
Commercial assay or kit In Situ Cell Death Detection Kit, Fluorescein Roche 11684795910
Commercial assay or kit DIG RNA Labeling Kit Roche 11277073910
Commercial assay or kit GFP Trap Agarose Chromotek gta-20
Commercial assay or kit Arcturus PicoPure RNA Isolation Kit Thermo Fisher Scientific KIT0204
Commercial assay or kit NEB Next Ultra II Directional RNA Library Prep Kit New England BioLabs E7760L
Chemical compound, drug Acridine Orange (AO) Nacalai tesque 1B-307 5 μg/ml
Chemical compound, drug CellTrace BODIPY TR Methyl Ester Thermo Fisher Scientific C34556 100 nM
Chemical compound, drug Ethyl-3-aminobenzoate de methanesulfonate (Tricaine, MS-222) Nacalai tesque 14805-82 0.02%
Chemical compound, drug PTU (N-Phenylthiourea) Nacalai tesque 27429-22 0.003%
Chemical compound, drug Fast DiO solid Thermo Fisher Scientific D3898 2 mg/ml
Chemical compound, drug Fast DiI solid Thermo Fisher Scientific D7756 2 mg/ml
Chemical compound, drug TO-PRO-3 Iodide (642/661) Thermo Fisher Scientific T3605 1 nM
Chemical compound, drug Hoechst 33,342 Wako 346-07951 1 ng/ml
Chemical compound, drug Toluidine Blue Nacalai tesque 1B-481
Chemical compound, drug Dextran, Tetramethylrhodamine Thermo Fisher Scientific D1817 LTJ
Chemical compound, drug Dextran, Alexa Flour-488 Thermo Fisher Scientific D22910
Chemical compound, drug Dextran, Alexa Flour-647 Thermo Fisher Scientific D22914
Chemical compound, drug Dextran, Cascade Blue Thermo Fisher Scientific D1976
Software, algorithm chopchop chopchop https://chopchop.cbu.uib.no
Software, algorithm ImageJ (Fiji)  PMID: 22930834 https://imagej.nih.gov/ij/; RRID: SCR_003070
Software, algorithm Imaris Bitplane http://www.bitplane.com/imaris; RRID: SCR_007370
Software, algorithm Proteome Discoverer Thermo https://www.thermofisher.com/store/products/OPTON-30945#/OPTON-30945
Software, algorithm STRING PMID: 27924014 https://string-db.org
Software, algorithm Metascape PMID: 30944313 https://metascape.org
Software, algorithm BioVenn PMID: 18925949 http://www.biovenn.nl/
Software, algorithm GraphPad Prism v9.1.0. GraphPad Prism https://www.graphpad.com/scientific-software/prism/