| Software, Algorithm |
TCRdist |
Dash et al., 2017, Bradley et al., 2017
|
|
Version 0.0.2; Software can be found on GitHub
|
| Software, Algorithm |
migec |
Shugay et al., 2014
|
RRID: SCR_016337
|
Version 1.2.9; Software can be found on GitHub
|
| Antibody |
CD3-PerCP eFluor710 (Mouse monoclonal) |
Thermo Fisher Scientific |
Cat: 46-0037-42; RRID: AB_1834395
|
0.012 μg per 1 million cells (1:100) |
| Antibody |
CD4-BV650 (Mouse monoclonal) |
BD Biosciences |
Cat: 563875; RRID: AB_2687486
|
2 μl per 1 million cells (1:50) |
| Antibody |
CD8-APC Fire750 (Mouse monoclonal) |
Biolegend |
Cat: 344746; RRID: AB_2572095
|
0.1 μg per 1 million cells (1:100) |
| Antibody |
TCRγ/-PE Cy7 (Mouse monoclonal) |
Biolegend |
Cat: 331222; RRID: AB_2562891
|
1 μg per 1 million cells (1:40) |
| Other |
Fc Block |
BD Biosciences |
Cat: 564220; RRID: AB_2728082
|
2.5 μg per 1 million cells (1:20) |
| Other |
Live/Dead Aqua |
Tonbo Biosciences |
Cat: 13–0870 T100 |
1 μl per 1 million cells (1:100) |
| Commercial assay, kit |
Qiagen QIAamp DNA Mini Kit |
Qiagen |
Cat: 51,306 |
|
| Commercial assay, kit |
Taqman SNP Genotyping Assay |
Thermo Fisher Scientific |
Cat: 4351379 |
|
| Commercial assay, kit |
TaqMan Genotyping Master Mix |
Thermo Fisher Scientific |
Cat: 4371353 |
|
