FIGURE 2.

Few thylakoid proteins are affected by the loss of LHCB1. The accumulation of proteins was assessed in 14-days-old seedlings of WT and L1ko lines by immunoblotting. The relative protein accumulation, normalized over the actin, was measured compared to the WT level. Each point represents the quantification of the relevant protein in an individual biological replicate; we analyzed one sample per genotype for proteins with low variation between lines and two or three samples for proteins with larger variation. The blue dot represents the average and the blue bars the standard error of the distribution. The proteins were divided into the complexes to which they are associated (LHCII, PSII, cytb6f, PSI, LHCI, and ATPsyn). The accumulation kinases and phosphatase mainly involved in the regulation of the phosphorylation of LHCII and PSII were also measured. The measured levels were compared to the average of 1, corresponding to the protein accumulation in WT extracts via a Student’s t-test; asterisks indicate p-value thresholds (0.0001 < p < 0.001 “***”, 0.001 < p < 0.05 “**”, 0.05 < p < 0.10 “*”, 0.10 < p “ns”) (see Supplementary Figures 3A,B).