Table 4.
Stability of surface antigens on plasma cells
| Markers | Fold change (SD)a |
||
|---|---|---|---|
| 24 hours | 48 hours | 72 hours | |
| A. 25°C | |||
| CD38 FITC | 0.93 (0.09) | 0.86 (0.16) | 0.84 (0.20) |
| p-value | NS | NS | NS |
| CD229 PE | 1.01 (0.16) | 1.06 (0.31) | 1.02 (0.37) |
| p-value | NS | NS | NS |
| CD319 APCb | 1.05 (0.08) | 1.03 (0.04) | 1.12 (0.07) |
| p-value | NS | NS | 0.041 |
| CD138 BUV395b | 0.91 (0.14) | 0.85 (0.30) | 0.69 (0.11) |
| p-value | NS | NS | 0.008 |
|
| |||
| B. 4°C | |||
| CD38 FITC | 0.98 (0.05) | 0.98 (0.07) | 0.99 (0.12) |
| p-value | NS | NS | NS |
| CD229 PE | 1.09 (0.16) | 1.13 (0.14) | 1.13 (0.23) |
| p-value | NS | NS | NS |
| CD319 APC | 1.01 (0.11) | 0.98 (0.05) | 1.02 (0.06) |
| p-value | NS | NS | NS |
| CD138 BUV395b | 0.62 (0.24) | 0.33 (0.26) | 0.26 (0.22) |
| p-value | 0.054 | 0.0120 | 0.004 |
Bone marrow aspirates from patients with plasma cell neoplasms (n = 3) were stored in RPMI 1640 at 4°C and 25°C over a period of 72 hours. An aliquot was stained every 24 hours for flow cytometric assessment. The expression levels of CD38, CD229, CD319, and CD138 on plasma cells were assessed.
a
Measured fold change was normalized to fresh bone marrow samples stained within 3 hours of collection.
b
Bold: antigens with expression level altered significantly from baseline
NS: not significant.