Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 17;8(3):245–256. doi: 10.1038/s41477-022-01108-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 1 — The workflow illustrating the procedures involved in the generation of transgenic TALEN-expressing lines and the detection of TALEN cleavage activity in the mitochondrial genome is schematically shown. Tobacco leaves are transformed with Agrobacterium tumefaciens cells harboring TALEN-encoding plasmids. Putative transgenic T₀ shoots are regenerated on medium containing kanamycin, rooted and transferred to the greenhouse. DNA is isolated from these plants and assayed by Southern blotting for TALEN activity (that is, cleavage of nad9). Individual T₁ seedlings derived from these plants (and selected for kanamycin resistance) are screened again for TALEN activity and then used for T₂ seed production. The blue-white pill indicates presence of kanamycin in the medium.