FIG. 1.

Treatment schedules of HBV baculovirus-infected HepG2 cells with 3TC prior to HBV RNA and DNA analyses. HepG2 cells were infected with HBV baculovirus and treated with 3TC according to three treatment schedules. For the first schedule (P), 3TC treatment was initiated 16 h prior to HBV baculovirus infection (pretreatment); in this group 3TC treatment was continued during and after infection. For the second schedule (T), 3TC treatment was initiated 24 h p.i. For the third treatment schedule (T*), 3TC was initiated 4 days after HBV baculovirus infection. On the indicated days (arrows), HBV replication in HepG2 cultures was assessed by examining intracellular and extracellular HBV DNA and HBV RNA by Southern and Northern blotting, respectively.