FIG. 2.

Analysis of HBV DNA secreted by HepG2 cells infected with 50 PFU of HBV baculovirus/cell and treated with increasing concentrations of 3TC over a 10-day period. Treatments with 0, 0.02, 0.2, and 2.0 μM concentrations of 3TC were initiated either 16 h prior to HBV baculovirus infection (P) or 24 h p.i. (T). At 4, 7, and 10 days p.i., DNA was extracted from the medium of each culture and analyzed by Southern blotting. RC and DS forms of HBV DNA are indicated. The percentages of HBV DNA present in treated cultures relative to untreated controls are indicated.