FIG. 3.

Analysis of HBV replicative intermediates in HepG2 cells infected with 50 PFU of HBV baculovirus/cell and treated with increasing concentrations of 3TC over a 10-day period. Treatments with 0, 0.02, 0.2, and 2.0 μM concentrations of 3TC were initiated either 16 h prior to HBV baculovirus infection (P) or 24 h p.i. (T). At 4, 7, and 10 days p.i., replicative intermediates were extracted from cytoplasmic core particles isolated from each culture. Replicative intermediates were analyzed by Southern blotting. RC, DS, and SS forms of HBV genomic DNA are indicated. The percentages of HBV DNA present in treated cultures relative to untreated control cultures are indicated.