FIG. 4.

Analysis of CCC HBV DNA produced in HepG2 cells infected with 50 PFU of HBV baculovirus/cell and treated with increasing concentrations of 3TC over a 7-day period. Treatments with 0, 0.02, 0.2, and 2.0 μM concentrations of 3TC were initiated either 16 h prior to HBV baculovirus infection (P) or 24 h p.i. (T). At 4 and 7 days p.i., non-protein-associated DNA was extracted from each culture and analyzed by Southern blotting. RC and CCC forms of HBV DNA are indicated. The percentages of HBV RC DNA and the percentages of CCC HBV DNA present in treated cultures relative to untreated control cultures are indicated.