FIG. 5.

Analysis of HBV transcripts in HepG2 cells infected with 50 PFU of HBV baculovirus/cell and treated with increasing concentrations of 3TC over a 7-day period. Treatments with 0, 0.02, 0.2, and 2.0 μM concentrations of 3TC were initiated either 16 h prior to HBV baculovirus infection (P) or 24 h p.i. (T). At 4 and 7 days p.i., total RNA was harvested from cultures and 20 μg of total RNA was analyzed by Northern blotting. The 3.5-, 2.4-, and 2.1-kb HBV transcripts are indicated.