FIG. 7.

Effect of 0.2 μM 3TC on the HBV DNA content of media from HepG2 cells expressing increasing levels of HBV. HepG2 cultures were infected with 25, 50, 100, 200, or 400 PFU of HBV baculovirus/cell and were treated with 0.2 μM 3TC starting 24 h p.i. Cultures were fed a fresh 3TC-supplemented medium daily. After 3 days of treatment (4 days p.i.), DNA was extracted from the media of treated and untreated control cultures and analyzed by Southern blotting. The autoradiograms shown indicate different lengths of time of exposure of the blot on film: 8 h (A), 4 h (B), 2 h (C), and 1 h (D). RC and DS forms of HBV DNA are indicated.