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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Genesis. 2022 Feb 18;60(3):e23468. doi: 10.1002/dvg.23468

Table 1.

Genotyping results and recombination patterns for Wnt1-Cre2 reporter crosses

♂ROSA26mTmG/+; Wnt1-Cre2Tg/+ × ♀Wild Type n=6 Sires
Genotype Expected Pattern Germline Pattern Total Embryos # Expected Pattern (%) # Germline Pattern (%)
ROSA26+/+;
Wnt1-Cre2+/+ 		Not Fluorescent Not Fluorescent 34 34 (100) N/A
ROSA26+/+;
Wnt1-Cre2Tg/+ 		Not Fluorescent Not Fluorescent 29 29 (100) N/A
ROSA26mTmG/+;
Wnt1-Cre2+/+ 		All tdTomato+ All EGFP+ 25 0 (0) 25 (100)
ROSA26mTmG/+;
Wnt1-Cre2Tg/+ 		EGFP+ Neural Crest All EGFP+ 28 0 (0) 28 (100)
♂Wild Type x ♀ROSA26mTmG/+; Wnt1-Cre2Tg/+ n=6 Dams
Genotype Expected Pattern Germline Pattern Total Embryos # Expected Pattern (%) # Germline Pattern (%)
ROSA26+/+;
Wnt1-Cre2+/+ 		Not Fluorescent Not Fluorescent 18 18 (100) N/A
ROSA26+/+;
Wnt1-Cre2Tg/+ 		Not Fluorescent Not Fluorescent 13 13 (100) N/A
ROSA26mTmG/+;
Wnt1-Cre2+/+ 		All tdTomato+ All EGFP+ 15 15 (100) 0 (0)
ROSA26mTmG/+;
Wnt1-Cre2Tg/+ 		EGFP+ Neural Crest All EGFP+ 14 14 (100) 0 (0)

When Wnt1-Cre2 and a floxed reporter coexist in the male line, 100% germline recombination is observed in ROSA26mTmG/+ offspring, even whenWnt1-Cre2 is not coinherited (25/25 Cre− embryos and 28/28 Cre+ embryos), indicating germline activity for this strain. However, germline activity is not observed when the genes are inherited through the female lineage.