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. 2021 Dec 8;16(4):1176–1186. doi: 10.1038/s41396-021-01157-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A The biomass of 24 h-old biofilms of P. aeruginosa MPAO1 transposon mutants grown in M9 medium in 96-well plates was quantified by crystal violet staining. Results represent the mean ± SD of two biological repeats with eight technical repeats each. Student’s t-tests were performed with ** equals to P < 0.01 and *** equals to P < 0.001. Tolerance of biofilm cells to B colistin and C gentamicin was assessed by measuring the optical density of the supernatant after the recovery of treated biofilm cells compared to non-treated ones (defined as 100%). Results represent the mean ± SD of two biological repeats (three for arnB and flgE mutants) with four technical repeats each. Student’s t-tests were performed in comparison to the condition without antibiotics for each mutant with * equals to P < 0.05 and *** equals to P < 0.001.