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. 2021 Dec 8;16(4):1176–1186. doi: 10.1038/s41396-021-01157-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — RNA was extracted from 24 h-old biofilms, grown in the same conditions as Fig. 2, and the expression of rpsL, pelA, pelB, pslA, pslB, and cdrA genes were quantified by qPCR. Expression fold change were normalized according to the expression of the housekeeping gene rpsL in MPAO1 WT (defined as 1) and in ΔflgE biofilms. Results represent the mean ± SD of three measurements done on two independent RNA extractions. Student’s t-tests were performed in comparison to the mRNA levels in MPAO1 WT with ***P < 0.001. All primers used are described in Table S1.