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. 2022 Mar 22;12:4893. doi: 10.1038/s41598-022-08870-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Genetic labeling of Egr2-expressing cells and progenies in mammary gland. (A) Experimental design. (B) Whole-mount imaging of GFP and tdTomato fluorescence in mammary gland from 3-week-old Egr2-Cre;ROSA26^mTmG or ROSA26^mTmG mice. Scale bar = 500 μm. (C, D) Representative flow profiles (C) and summary bar plots (D) displaying the number of GFP-positive cells in basal and luminal cells during pubertal mammary development. p value was generated using a Student’s t-test. n = 3 Egr2-Cre;ROSA26^mTmG mice per age analyzed.