Binding affinity of SQ and its glycosides for SmoF. a) Isothermal titration calorimogram showing titration of SQ into SmoF. b) Calorimogram of SQMe into SmoF. c) Melting temperature (Tm) of SmoF, as determined by differential scanning fluorimetry, the Tm shift relative to apo-SmoF, and Kd values determined by ITC for SQ, SQMe, SQDG-(C4:0/C16:0) and SQDG-(C18:1/C16:0). Dissociation constants for SQDG-(C4:0/C16:0) and SQDG-(C18:1/C16:0) could not be measured (noted by a dash). Data for SQGro (in blue) was reported in (Sharma et al., 2022) and has been included for comparison. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)