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. 2021 Nov 6;16(4):958–971. doi: 10.1038/s41396-021-01144-0

Fig. 2. Activity-based fluorescent labeling of catalytically active ammonia-, methane-, and other alkane-oxidizing microorganisms.

Fig. 2

Fluorescent micrographs of 1,7OD pre-incubated cells of a Nitrosomonas europaea, b Nitrospira inopinata, c Methylotetracoccus oryzae, d Methylosinus sporium M29, e Methylacidiphilum fumariolicum SolV, f Methylocella tundrae, g Rhodococcus sp. strain ZPP, and h Thauera butanivorans. Cells were stained with the activity-based labeling protocol (shown in green) and FISH probes specific for a AOB (Nso190, Neu653, Nso1225; red), b Nitrospira (Ntspa662, Ntspa712; red), c, f Gammaproteobacteria (Gam42a; red), d Alphaproteobacteria (Alf0001b, Alf0968; red), e Verrucomicrobia (EUB338III; red), h Betaproteobacteria (Bet42a; red), and a–f, h) all bacteria (EUB338mix; blue). Cells in g were counterstained with DAPI (blue). Scale bars correspond to 20 µm.