Skip to main content
. 2021 Nov 6;16(4):958–971. doi: 10.1038/s41396-021-01144-0

Fig. 6. Activity-based fluorescent labeling of catalytically active ammonia-oxidizing bacteria present in complex microbial communities.

Fig. 6

Fluorescent micrographs showing a, b a culture containing Ca. N. nitrosa and Ca. N. nitrificans and c, d a nitrifying enrichment culture pre-incubated (a, c) with and (b, d) without 1,7OD (control). Cells are stained with the activity-based labeling protocol (shown in green) and FISH probes specific for Nitrospira (Ntspa662, Ntspa712; shown a, b in red and c, d in blue), c, d ammonia-oxidizing bacteria (Nso190, NEU653, Nso1225; in red), and a, b all bacteria (EUB338mix; in blue). Scale bars correspond to 10 µm.