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. 2022 Mar 22;12:4853. doi: 10.1038/s41598-022-08949-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Anti-CD80/86 antibodies suppress T lymphocyte proliferation in mixed lymphocyte reaction assay. Representative flow cytometry plots of CD4⁺ (a) CFSE-labeled cells. (b) The frequency of CD4⁺ CFSE^low cells in CD4⁺ T cells was significantly reduced in the allogeneic stimulation group with anti-CD80/86 antibodies compared with that in without anti-CD80/86 antibodies group (n = 3, ***P < 0.001). (c) Representative columns of BrdU assay results. Anti-CD80/86 suppressed T cell proliferation compared to without anti-CD80/86 antibodies in allogeneic stimulation and no allogeneic stimulation (n = 3, **P = 0.007, *P = 0.019, respectively). (d) Representative flow cytometry plots of the frequency of IFN-γ⁺CD4⁺ T lymphocyte with or without allogeneic stimulation and anti-CD80/86 intervention. (e) Frequency of IFN-γ⁺CD4⁺ T lymphocyte in CD4⁺ T cells reduced following treatment with anti-CD80/86 antibodies compared with that without anti-CD80/86 antibody treatment in allogeneic stimulation (n = 3, * P < 0.001). Data are presented as mean ± SEM. CFSE carboxyfluorescein diacetate succinimidyl ester.