Table 1.

This table illustrates the similarities and differences in behavior observed in constitutive versus conditional knockout models of Gabrb3 illustrating cell-type specific roles in behavioral diversity.

Animal models	Constitutive Gabrb3 Knockout	Conditional Gabrb3 Knockout; Neuron-specific	Conditional Gabrb3 Knockout; Forebrain-specific	Conditional Gabrb3 Knockout; endothelial cell-specific	Constitutive Gabrb2 Knockout
Gene targeting	Targeted disruption of the β3 locus; Exons 1–3	Use of Synapsin 1-cre Target: Exon 3	Use of CamKII-cre Target: Exon 3	Use of Tie2-cre Target: Exon 3	Targeted disruption of the β2 locus; Exons 6–7
References	Homanics et al., 1997; De Lorey et al.; 1998	Ferguson et al., 2007	Ferguson et al., 2007	Li et al. 2018; Subburaju et al., 2020	Yeung et al., 2018
Time of gene inactivation	Embryonic Day 0	Embryonic Day 12.5+	~ 2 weeks postnatal	Embryonic Day 9+	Embryonic Day 0
Tissue specificity	All cells	Most neurons	Primarily forebrain neurons	Endothelial cells	All cells
Altered behaviors	Impaired cognitive functions Increased locomotor activity Hyperactive-intense circling pattern Stereotypical behavior	Cognitive functions not tested Locomotor activity not tested No hyperactivity Not reported	Normal cognitive functions Increased locomotor activity Hyper-responsive to human contact Not reported	Impaired cognitive functions-mild Increased locomotor activity Hyperactive-continuous running Stereotypical Behavior	Impaired cognitive functions Increased locomotor activity Hyperactive movement Stereotypical Behavior
Other behaviors reported	Reduced Etomidate LORR Impaired maternal behavior; foot clasping behavior EEG abnormalities, disturbed rest-activity cycle	Reduced Etomidate LORR Normal maternal behavior; no foot clasping behavior No seizure like activity observed	Reduced Etomidate LORR Impaired maternal behavior; no foot clasping behavior EEG: not tested	Increased anxiety and depression Impaired social communication and social novelty Impaired prepulse inhibition of startle	Reduced affective symptoms Impaired social affiliation and social novelty Impaired prepulse inhibition of startle
Body size	Runted until weaning	Normal	Some become obese	Runted until weaning	Normal
Neonatal lethality	90% die as neonates	61% die as neonates	30% die at P15-P25	No pup mortality	No pup mortality
Seizure-like activity	Seizures (epilepsy) Multiple seizure types	No tremors or seizures	Occasional absence-like and convulsive seizures	Seizure-like symptoms in 15% mice	Accelerated PTZ-induced seizure; susceptible to audiogenic epilepsy
Disease similarity	Angelman syndrome or autism spectrum disorder (ASD)	Unclear	ASD	Anxiety, Depression, Schizophrenia,	Schizophrenia