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. 2022 Mar 22;12:4344. doi: 10.1038/s41598-022-08583-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of the mitochondrial function of MITO cells by measuring extracellular flux analyzer. The extracellular flux analyzer provides a continuous quantitative assessment of mitochondrial respiration in CPCs by measuring oxygen consumption. The Seahorse FXp was used to measure the mitochondrial oxygen consumption rate (OCR) of CPCs based on the protocol shown in (A)(a). Basic OCR, ATP-linked OCR, maximum OCR and non-mitochondrial OCR were measured. These parameters were estimated by the sequential addition of oligomycin (an inhibitor of ATP synthesis), carbonyl cyanide p-trifluoromethoxy-phenyl-hydrazone (FCCP) (a mitochondrial inner membrane decarboxylator that maximizes the mitochondrial electron transfer system), and rotenone and antimycin A (the complete inhibition of mitochondrial respiration) and the OCRs were measured (B). Significant differences were calculated using one-way ANOVA followed by the SNK test (**p < 0.01) (n = 3).