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. 2022 Mar 22;12:4344. doi: 10.1038/s41598-022-08583-5

Figure 7.

Figure 7

Detection of oxidative stress of ischemic myocardium after cell transplantation. (A) CLSM observation of oxidative stress of ischemic myocardium 3 days after treatment in each group using DHE (a red dye for reactive oxygen spices). (a) Sham group, (b) PBS group, (c) CPC transplantation group and (d) MITO-cell transplantation group. Scale bars, 200 μm. (B) Quantification of relative DHE positive region ratio was estimated using CLSM images. Data are represented as the mean ± S.D (sham group (n = 6), PBS group (n = 4), CPC transplantation group (n = 4) and MITO-cell transplantation group (n = 4)). Significant differences (*p < 0.05, **p < 0.01) were calculated by one-way ANOVA, followed by SNK test. Scale bar is 200 μm. Detection of mitochondrial membrane potential of ischemic myocardium after cell transplantation. (C) CLSM observation of mitochondrial membrane potentials of ischemic myocardium 3 days after treatment in each group using TMRM (a red dye for mitochondrial membrane potential). (a) Sham group, (b) PBS group, (c) CPC transplantation group and (d) MITO-cell transplantation group. Scale bars 200 μm. (D) Quantification of relative TMRM positive region ratio was estimated using CLSM images. Data are represented as the mean ± S.D (sham group (n = 6), PBS group (n = 5), CPC transplantation group (n = 5) and MITO-cell transplantation group (n = 5)). Significant differences (*p < 0.05, **p < 0.01) were calculated by one-way ANOVA, followed by SNK test.