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. 2022 Mar 22;12:114. doi: 10.1038/s41398-022-01875-4

Fig. 1. Prenatal exposure to VPA caused the alterations of PGRN temporal expression in the cerebellum.

Fig. 1

Representative blots (A) and quantification (B) showed the expression of PGRN at four different time points. Representative images (bar = 100 μm) (C) and quantification (D) of immunofluorescence staining described that the expression of PGRN is in the same trend as Western blotting. E PGRN ELISA protein quantitation of cerebellar tissue. Data are expressed as mean ± SEM. Two-way ANOVA followed by Sidak post hoc. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, sample sizes (n): n = 5/group for Western blotting and immunofluorescence staining. n = 4/group for ELISA).