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Immunofluorescence evaluation of NSCs and its differentiation in vitro. (A) Fluorescence images of NSCs differentiation in different hydrogels into Tuj-1+ and GFAP+ cells on day 7. (B, C) Fluorescence quantitative analysis of Tuj-1 and GFAP. (D and E) mRNA expression analysis of Tuj-1 and GFAP. (F) Fluorescence images of NSCs differentiation in different hydrogels into MAP-2+ cells on day 7. (G) Fluorescence quantitative analysis of MAP-2. (H) mRNA expression analysis of MAP-2.
