Fig. 6.

Immunofluorescence evaluation of NSCs and its differentiation in vivo. (A) Representative images of Nestin and GFAP immunostaining of spinal sections in SCI, AG and AGP3 groups at 2 weeks post-surgery. (B) Representative images of GFAP and Tuj-1 immunostaining of tissue samples at 6 weeks after SCI. (C) Images of GFAP and MAP-2 immunostaining of spinal sections in different groups at 6 weeks after injury. (D) Quantification of relative intensities of Nestin⁺ cells in the lesion site. (E) Quantification of regenerated Tuj-1⁺ neurons in different groups. (F) Quantification of relative intensities of MAP-2⁺ neurons. (G) Representative images of EdU and Tuj-1 immunostaining of tissue samples. (H) Representative confocal Z-stack images showing EdU and Tuj-1 colocalization in the AGP3 group. (I) Representative images of EdU and NeuN immunostaining of spinal sections in different groups. (J) Representative confocal Z-stack images showing EdU and NeuN colocalization in the AGP3 group. (K) Quantification of relative intensities of EdU⁺ neuron.