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. 2021 Dec 23;15:103–119. doi: 10.1016/j.bioactmat.2021.11.032

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 9 — Assessments of intracellular calcium of NSCs treated with antagonist. (A, B) Immunofluorescence images of L-VGCC, p-CREB and BDNF in each group on day 7. (C) WB analysis of L-VGCC, p-CREB and BDNF. (D) Quantitative analysis of L-VGCC, p-CREB and BDNF. (E) Representative pseudo-color images in nifedipine-treated cells before and after glutamate stimulation. (F) Image-derived fluo-4 intensity measurements over time in nifedipine-treated NSCs. (G) Schematic diagram showing the possible signal transduction pathways of the promoted neurogenesis.