| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-BrdU-Alexa Fluor® 488 (BrdU Monoclonal Antibody MoBU-1, Alexa Fluor 488) | ThermoFisher Scientific (Invitrogen) | Cat #B35130; RRID: AB_2536434 |
| Mouse anti-glutamine synthetase (GS) | BD Biosciences | Cat #610517; RRID: AB_2313837 |
| Rat anti-BrdU-Alexa Fluor® 555 | Abcam | Cat #ab221240; RRID: AB_2893129. |
| Rabbit anti-SIFamide | Gift from Dr. A. Yasuda (Suntory Institute for Bioorganic Research, Osaka Japan) Yasuda et al., 2004; Yasuda-Kamatani and Yasuda, 2006; Polanska et al., 2007 |
N/A |
| AffiniPure goat anti-rabbit IgG (H+L) (minus x human, mouse, rat serum proteins) conjugated to Cy5 | Jackson ImmunoResearch Laboratories | Cat #111-175-144; RRID: AB_2338013 |
| Goat anti-mouse IgG DyLight 649 | Jackson ImmunoResearch Labs | Cat#205-492-176; RRID: AB_2339069 |
| Chemicals, peptides, and recombinant proteins | ||
| 5-bromo-2’-deoxyuridine (BrdU) | Millipore Sigma | Cat #19-160 |
| 4% paraformaldehyde | Fisher Scientific (EM Sciences) | Cat #50-259-99 |
| Triton X-100 | Sigma-Aldrich | Cat #X100 |
| Propidium iodide | ThermoFisher (InvitrogenTM) | Cat #P3566 |
| Hoechst 33342 | ThermoFisher Scientific | Cat #H3570 |
| DAPI | ThermoFisher Scientific | Cat #62248 |
| Trypan blue, 4% solution | ThermoFisher Scientific | Cat #15250061 |
| Serotonin creatinine sulfate | Millipore Sigma | Cat #H7752 |
| Fluoro-Gel | EM Sciences | Cat #17985-10 |
| Recombinant-astakine 1 protein (r-AST1) | Gift from Irene Söderhäll (Uppsala University, Sweden). Method in Lin et al., 2010 | N/A |
| Percoll Plus | GE Healthcare Life Sciences (now Cytiva) | Cat #17544501 |
| Critical commercial assays | ||
| CLICK-iTTM EdU Cell Proliferation Kit for Imaging, Alexa FluorTM 488 dye | ThermoFisher Scientific (Invitrogen) | Cat #C10337 |
| Experimental models: Organisms/strains | ||
| Procambarus clarkii (red swamp crayfish) | Atchafalaya Biological Supply Co | N/A |
| Procambarus clarkii (red swamp crayfish) | Carolina Biological Supply Co. | N/A |
| Software and algorithms | ||
| JMP Data Analysis Software | SAS Institute | https://www.jmp.com/en_us/software/data-analysis-software.html |
| IBM SPSS Statistics | IBM Corp |
https://www.ibm.com/analytics/spss-statistics-software RRID: SCR_019096 |
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