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. 2022 Mar 8;25(4):104038. doi: 10.1016/j.isci.2022.104038

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Zeb1 promotes BMC mobility in vitro

Monolayer-cultured BMCs were first treated with mitomycin for 1 h to completely stop cell proliferation and then 3 straight scratch lines were created with a 200-μL pipet tip on each culture plate.

(A and B) The gap width of cultured Zeb1^+/+ and Zeb1^−/+ BMCs isolated from animals with and without alkali burn was photographed and measured every day for 3 days using ImageJ.

(C) Representative images of the monolayer-cultured Zeb1^+/+ and Zeb1^−/+ BMCs in 0 and 3 days after the straight line was made.

(D) Expression of the cytoskeletal genes vimentin (Vim), actin alpha 1 (Acta1), and integrin alpha 3 (Itga3) in BMCs isolated from control animals without alkali burn. Data are represented as mean ± standard deviation. Ctrl, PBS control; AB, alkali burn; Zeb1 wild type, Zeb1^+/+; Zeb1 het, Zeb1^−/+; ∗, p < 0.05; ∗∗, p < 0.01.