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. 2022 Mar 8;25(4):104038. doi: 10.1016/j.isci.2022.104038
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Rabbit polyclonal Zeb1 anti-serum Dr. Douglas Darling N/A
Rabbit polyclonal Arg1 antibody Novus Biologicals Cat.# NBP1 - 32731
Goat polyclonal IL-6 antibody Santa Cruz Biotechnology Cat.# sc-1265
Rat monoclonal CD45 antibody eBioscience Cat.# 14-0451-81
Rabbit polyclonal NF-κB (p65) antibody Cell Signaling Cat.# 8242T
Rabbit polyclonal p-NF-κB (p65) antibody Abclonal Cat.# AP0123
Mouse monoclonal Stat3 antibody Cell Signaling Cat.# 9139S
Mouse monoclonal p-Stat3 (Tyr705) antibody Cell Signaling Cat.# 4113S
Mouse monoclonal β-Actin antibody Sigma Cat.# A1978
Mouse monoclonal CD11b antibody eBioscience Cat.# RM2820
Rabbit monoclonal H3 antibody Abcam Cat.# ab176842

Chemicals, peptides, and recombinant proteins

Lipopolysaccharide (LPS) Sigma-Aldrich Cat.# L6529
Transforming growth factor beta (TGFb) R&D Systems Cat.# 240-B
Mitomycin C Sigma-Aldrich Cat.# M5353
Trypan blue solution Sigma-Aldrich Cat.# 93595

Critical commercial assays

Red blood cell (RBC) lysis buffer eBioscience Cat.# 00-4333-57
Enhanced chemiluminescence (ECL) detection reagents Cytiva Cat.# RPN2134
Nuclear dye Hoechst 33342 ThermoFisher Cat.# 62249

Experimental models: Cell lines

Mouse embryonic fibroblasts (MEF) of both Zeb1 wildtype (Zeb1+/+) and knockout (KO, Zeb1−/−) This paper N/A
Mouse bone marrow-derived cells (BMC) of both Zeb1 wildtype (Zeb1+/+) and heterozygous (Zeb1-/+) This paper N/A

Experimental models: Organisms/strains

Zeb1tm1Yhi/Zeb1tm1YhiMus musculus
Genetic Background: B6.129P2-Zeb1
Mouse Genome Informatics (MGI) RRID:MGI:3653696

Oligonucleotides

Primers, see Tables S1 and S2 This paper N/A

Software and algorithms

ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/
FlowJo Tree Star Inc. http://www.treestar.com/