REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Rabbit polyclonal Zeb1 anti-serum | Dr. Douglas Darling | N/A |
Rabbit polyclonal Arg1 antibody | Novus Biologicals | Cat.# NBP1 - 32731 |
Goat polyclonal IL-6 antibody | Santa Cruz Biotechnology | Cat.# sc-1265 |
Rat monoclonal CD45 antibody | eBioscience | Cat.# 14-0451-81 |
Rabbit polyclonal NF-κB (p65) antibody | Cell Signaling | Cat.# 8242T |
Rabbit polyclonal p-NF-κB (p65) antibody | Abclonal | Cat.# AP0123 |
Mouse monoclonal Stat3 antibody | Cell Signaling | Cat.# 9139S |
Mouse monoclonal p-Stat3 (Tyr705) antibody | Cell Signaling | Cat.# 4113S |
Mouse monoclonal β-Actin antibody | Sigma | Cat.# A1978 |
Mouse monoclonal CD11b antibody | eBioscience | Cat.# RM2820 |
Rabbit monoclonal H3 antibody | Abcam | Cat.# ab176842 |
Chemicals, peptides, and recombinant proteins | ||
Lipopolysaccharide (LPS) | Sigma-Aldrich | Cat.# L6529 |
Transforming growth factor beta (TGFb) | R&D Systems | Cat.# 240-B |
Mitomycin C | Sigma-Aldrich | Cat.# M5353 |
Trypan blue solution | Sigma-Aldrich | Cat.# 93595 |
Critical commercial assays | ||
Red blood cell (RBC) lysis buffer | eBioscience | Cat.# 00-4333-57 |
Enhanced chemiluminescence (ECL) detection reagents | Cytiva | Cat.# RPN2134 |
Nuclear dye Hoechst 33342 | ThermoFisher | Cat.# 62249 |
Experimental models: Cell lines | ||
Mouse embryonic fibroblasts (MEF) of both Zeb1 wildtype (Zeb1+/+) and knockout (KO, Zeb1−/−) | This paper | N/A |
Mouse bone marrow-derived cells (BMC) of both Zeb1 wildtype (Zeb1+/+) and heterozygous (Zeb1-/+) | This paper | N/A |
Experimental models: Organisms/strains | ||
Zeb1tm1Yhi/Zeb1tm1YhiMus musculus Genetic Background: B6.129P2-Zeb1 |
Mouse Genome Informatics (MGI) | RRID:MGI:3653696 |
Oligonucleotides | ||
Primers, see Tables S1 and S2 | This paper | N/A |
Software and algorithms | ||
ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
FlowJo | Tree Star Inc. | http://www.treestar.com/ |