Antibodies |
|
Rabbit polyclonal anti-Synapsin1/2 |
Synaptic Systems |
Cat# 106 002, RRID:AB_887804
|
rbMuSK |
Dr. Markus A. Rüegg |
194T/Nsk-2 |
Rabbit polyclonal anti-Neurofilament 200 |
Sigma-Aldrich |
Cat# N4142, RRID:AB_477272
|
Mouse monoclonal anti-β-catenin |
Cell Signaling Technology |
Cat# 2698, RRID:AB_1030945
|
Mouse monoclonal anti-active β-catenin |
Millipore |
Cat# 05-665, RRID:AB_309887
|
Mouse monoclonal anti-Yap |
Santa Cruz Biotechnology |
Cat# sc-101199, RRID:AB_1131430
|
Rabbit monoclonal anti-Phospho-Yap (Ser127) |
Cell Signaling Technology |
Cat# 13008, RRID:AB_2650553
|
Mouse alpha Tubulin |
Abcam |
Cat# ab7291, RRID:AB_2241126
|
|
Chemicals, peptides, and recombinant proteins |
|
α-Bungarotoxin Alexa488-conjugate |
Invitrogen |
Cat# B13422
|
α-Bungarotoxin Alexa594-conjugate |
Invitrogen |
Cat# B13423
|
|
Critical commercial assays |
|
RNeasy Fibrous Tissue Mini Kit |
Qiagen |
Cat# 74704 |
SuperScript II Reverse Transcriptase |
Invirtogen |
Cat# 100004925 |
TaqMan Universal Master Mix |
Applied Biosystems |
Cat# 4440040 |
|
Experimental models: Organisms/strains |
|
Mouse: Dysgenic (CaV1.1+/-) |
Tanabe et al., 1988 |
NA |
Mouse: B6.129-Ctnnb1tmKem/KnwJ |
The Jackson Laboratory |
JAX: 004152 |
Mouse: B6.Ct-Tg(ACTA1-cre)79Jme/J |
The Jackson Laboratory |
JAX:006149 |
Mouse: Tg(TCF/Lef1-HIST1H2BB/EGFP)61Hadj/J |
The Jackson Laboratory |
JAX:013752 |
|
Oligonucleotides |
|
CaV1.1 Primer Forward: GCT TTG CAG ATG TTC GGG AAG ATC GCC ATG |
Eurofins |
NA |
CaV1.1 Primer Reverse: GCA GCT TTC CAC TCA GGA GGG ATC CAG TGT |
Eurofins |
NA |
β-cat(floxed) Primer Forward: AAG GTA GAG TGA TGA AAG TTG TT |
Eurofins |
NA |
β-cat(floxed) Primer Reverse: CAC CAT GTC CTC TGT CTA TTC |
Eurofins |
NA |
HSA-cre Primer Forward: GCG GTC TGG CAG TAA AAA CTA TC |
Eurofins |
NA |
HSA-cre Primer Reverse: GTG AAA CAG CAT TGC TGT CAC TT |
Eurofins |
NA |
Internal Positive Control Primer Forward: CTA GGC CAC AGA ATT GAA AGA TCT |
Eurofins |
NA |
Internal Positive Control Primer Reverse: GTA GGT GGA AAT TCT AGC ATC ATC C |
Eurofins |
NA |
TCF/Lef:GFP Primer Forward: ACA ACA AGC GCT CGA CCA TCA C |
Eurofins |
NA |
TCF/Lef:GFP Primer Reverse: AGT CGA TGC CCT TCA GCT CGA T |
Eurofins |
NA |
|
Software and algorithms |
|
Image Studio Lite 5.0 |
LI-COR |
https://www.licor.com/bio/image-studio-lite/ (Discontinued) |
Las AF 2.6.3 |
Leica Microsystems CMS GmbH |
https://www.leica-microsystems.com/ |
Zen 3.2 (blue edition) |
Carl Zeiss Microscopy GmbH |
https://www.zeiss.com/ |
Design & Analysis Software 2.4.3 |
Thermo Fisher Scientific |
https://www.thermofisher.com/at/en/home/global/forms/life-science/quantstudio-6-7-pro-software.html |
GraphPad Prism 9 |
GraphPad |
https://www.graphpad.com/ |
Adobe Photoshop CS6 13.0.1 |
Adobe Systems Incorporated |
https://www.adobe.com/ |
MetaMorph 7.8.0.0 |
Molecular Devices |
https://www.moleculardevices.com/ |