Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 15;25(5):165. doi: 10.3892/mmr.2022.12681

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Wen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

PMC Copyright notice

Figure 3. — Mitochondrial calcium overload induced by OGD. (A) OGD-stimulated calcium overload in the mitochondria of N2a cells (scale bars, 5 µm). (B and C) Western blot analysis revealed that OGD upregulated GRP75 expression in (B) HT22 and (C) N2a cells. (D) Western blot analysis revealed that OGD upregulated GRP75 expression in the MCAO tissue. (E) Western blot analysis was used to determine HIF1α expression in N2a cells. Representative images are provided and quantitative results are expressed as the mean ± standard error of the mean of each group from three separate experiments. **P<0.01, ***P<0.001, ****P<0.0001 vs. Ctrl. GRP75, 75 kDa glucose-regulated protein; Ctrl, control; OGD, oxygen-glucose deprivation; MCAO, middle cerebral artery occlusion; HIF, hypoxia-inducible factor.