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. 2022 Mar 15;23(5):146. doi: 10.3892/ol.2022.13266

Figure 5.

Figure 5.

PLK1 is required for Huh7 cell proliferation. Knockdown efficiency of PLK1 confirmed using (A) reverse transcription-quantitative PCR and (B) western blot analysis. Cell proliferation rate analyzed using a Cell Counting Kit-8 assay after (C) transfection with siPLK1 or (D) treatment with 10 nM BI 2536 in Huh7 cells. (E) Decrease in the proliferation rate of Huh7 cells following PLK1 knockdown as indicated by EdU staining (scale bars, 50 µm). DNA content of Huh7 cell analyzed via flow cytometry 24 h after (F) transfection with siPLK1 or (G) treatment with 10 nM BI 2536. Results are provided in histograms. Quantified values are expressed as the mean ± standard deviation of three independent experiments. *P<0.05, **P<0.01, ***P<0.001 vs. siNC. PLK1, polo-like kinase 1; siRNA, small interfering RNA; siNC, negative control siRNA; siPLK1, siRNA targeting PLK1; PI, propidium iodide; EdU, 5-ethynyl-2′-deoxyuridine; OD450, optical density at 450 nm.