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. 2022 Mar 15;2022:1792412. doi: 10.1155/2022/1792412

Figure 2.

Figure 2

GAS5 stimulates NPC apoptosis and inhibits cell proliferation and ECM remodeling. (a, b) Western blot analysis of ECM metabolism-related and apoptosis-related proteins in NPCs, which was normalized to GAPDH. n = 3. (c) Viability of NPCs assessed by CCK-8 assay. n = 3. (d, e) TUNEL staining analysis of NPCs (×400). ∗p < 0.05 vs. HDNPCs treated with oe-NC and #p < 0.05 vs. HDNPCs treated with si-NC. The cell experiment was repeated independently 3 times. Data (mean ± standard deviation) were assessed by one-way ANOVA, followed by Tukey's post hoc test. Data among groups at different time points were assessed with repeated measures ANOVA, followed by Bonferroni test. The experiment was repeated 3 times.