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. 2022 Mar 15;2022:1792412. doi: 10.1155/2022/1792412

Figure 5.

Figure 5

GAS5 upregulates Ang-2 expression in NPCs through downregulating miR-17-3p expression. (a) The binding sites between Ang-2 and miR-17-3p predicted using RNA22 website (https://cm.jefferson.edu/rna22/Interactive/). (b) The luciferase activity of Ang-2 reporter plasmids determined by dual-luciferase reporter assay (∗p < 0.05 vs. HEK 293T cells treated with NC mimic). (c) Expression of Ang-2 in NPCs after miR-17-3p was upregulated or downregulated and determined by RT-qPCR, which was normalized to GAPDH. (d, e) Western blot analysis of Ang-2 protein in NPCs after miR-17-3p was upregulated or downregulated, which was normalized to GAPDH. (f) Correlation analysis of miR-17-3p expression and Ang-2 expression in NP tissues. (g) Expression of Ang-2 in NPCs after GAS5 was upregulated or downregulated and detected by RT-qPCR, which was normalized to GAPDH. (h, i) Western blot analysis of Ang-2 protein in NPCs after GAS5 was upregulated or downregulated, which was normalized to GAPDH. ∗p < 0.05 vs. HDNPCs treated with si-NC, #p < 0.05 vs. HDNPCs treated with oe-NC, and $p < 0.05 vs. HDNPCs treated with oe-GAS5. Data (mean ± standard deviation) were analyzed by one-way ANOVA, followed by Tukey's post hoc test, while data in (b) were analyzed by unpaired t test. The cell experiment was repeated independently 3 times.