Table 1 –

Isolation platforms for CTCs in PC

Technology	CTC definition	Application in PC
Antibody-based positive selection
CellSearch	EpCAM+, CD45−, CK8+, CK18+, CK19+, DAPI+	[57]
Adna Test	EpCAM+, PSA+/PSMA+/EGFR+	[15]
CTC-Chip	EpCAM+, specific antigen+	[89]
CTC-iChip	>3.8 μm size, EpCAM+	[90]
IsoFlux	CD45−, DAPI+, PanCK+, or EpCAM+	[91]
MagSweeper	EpCAM+, CD45−, DAPI +	[10,92]
CellCollector	EpCAM+, CD45−, PanCK+, PSA+, Hoechst+	[74]
NanoVelcro	EpCAM+, CD4−, PanCK+, morphological verification	[93]
Antibody-based negative selection
EasySep	CD45−	[94]
RosetteSep	CD45−, CD66b−, glycophorin A−, density	[74]
EPISPOT	CD45−, CD66b−, glycophorin A−, PSA+, FGF2+	[74]
CTC-iChip	CD45−, CD16−, CD66b−	[90]
Selection free
Epic Sciences	PanCK+, CD45−, DAPI+, AR+	[9]
AccuCyte	DAPI+, PanCK+, CD45−, CD66b−, CD11b−, CD14−, CD34−, EpCAM+	[95]
Physical properties
ApoStream	Dielectrophoretic field flow	[96]
Celsee Diagnostics	>7.5 μm, deformability	[97]
ISET	≥8 μm	[95]

CTC = circulating tumor cell; EGFR = epidermal growth factor receptor; PC = prostate cancer; PSA = prostate-specific antigen; PSMA = prostate-specific membrane antigen.

Positive (+) and/or negative (−) expression of different capture/detection antigens, or physical properties, is used as a criterion for CTC isolation by different technologies or platforms.