Proline85 in nsp 10 and valine393 in nsp 14 are conserved among IBV strains. Genome alignment of the full consensus-level genome sequences, downloaded from GenBank, of the following IBV strains with known in vivo phenotypes: the vaccine strains H120 (MN548287) and CR88 (MN548285), the attenuated strain Beau-CK (AJ311317), and the pathogenic strains QX (MN548289), Italy-02 (MN548288), M41-CK (MK728875.1), and D1466 (MN548286). Proline85 in nsp 10 and leucine393 in nsp 14 appear to be conserved among the IBV strains. Black box indicates the location of the substituted residues identified.