TABLE 3.
Recombinant IBVs based on M41-R containing modified nucleotides in nsps 10, 14, 15, and 16a
| IBV | Nsp |
Amino acid changes | |||
|---|---|---|---|---|---|
| 10 | 14 | 15 | 16 | ||
| rIBV M41-R | U (Leu) | C (Leu) | A (Ile) | A (Ile) | |
| rIBV M41-K | C (Pro) | G (Val) | U (Leu) | G (Val) | Nsp10L85P-Nsp14L393V-Nsp15I183L-Nsp16I209V |
| M41-CK | C (Pro) | G (Val) | U (Leu) | G (Val) | |
| M41R-nsp10rep | C (Pro) | C (Leu) | A (Ile) | A (Ile) | Nsp10L85P |
| M41R-nsp10.14rep | C (Pro) | G (Val) | A (Ile) | A (Ile) | Nsp10L85P-Nsp14L393V |
| M41R-nsp10.15rep | C (Pro) | C (Leu) | U (Leu) | A(Ile) | Nsp10L85P-Nsp15I183L |
| M41R-nsp10.16rep | C (Pro) | C (Leu) | A (Ile) | G (Val) | Nsp10L85P-Nsp16I209V |
| M41R-nsp10.14.15rep | C (Pro) | G (Val) | U (Leu) | A (Ile) | Nsp10L85P-Nsp14L393V-Nsp15I183L |
| M41R-nsp10.14.16rep | C (Pro) | G (Val) | A (Ile) | G (Val) | Nsp10L85P-Nsp14L393V-Nsp16I209V |
| M41R-nsp10.15.16rep | C (Pro) | C (Leu) | U (Leu) | G (Val) | Nsp10L85P-Nsp15I183L-Nsp16I209V |
| M41R-nsp14.15.16rep | U (Leu) | G (Val) | U (Leu) | G (Val) | Nsp14L393V-Nsp15I183L-Nsp16I209V |
a
Boldface type indicates nucleotides derived from the pathogenic M41 CK sequence (GenBank accession no. MK728875.1, underlining represents nucleotides present in the apathogenic M41-R sequence). “rep” in the name of the rIBV stands for replaced and indicates the nucleotide replaced in the M41-R backbone. M41-K represents an rIBV, M41R-nsp10.14.15.16rep, in which all four of the M41-R nucleotides are replaced.