TABLE 5.
Presence of IBV-derived RNA and infectious virusa
| Expt. | Group | IBV-derived RNA |
Infectious IBV |
||||
|---|---|---|---|---|---|---|---|
| 4 dpi | 6 dpi | 7 dpi | 4 dpi | 6 dpi | 7 dpi | ||
| 1 | Mock | 0/3 | 0/3 | 0/6 | 0/3 | 0/3 | 0/6 |
| M41-R | 0/3 | 0/3 | 0/6 | 0/3 | 0/3 | 0/6 | |
| M41R-nsp10rep | 1/3 | 0/3 | 4/6 | 0/3 | 0/3 | 2/6 | |
| M41R-nsp14.15.16rep | 1/3 | 0/3 | 1/6 | 1/3 | 0/3 | 2/6 | |
| M41-CK | 2/3 | 1/3 | 2/6 | 3/3 | 1/3 | 1/6 | |
| 2 | Mock | 0/3 | 0/3 | 0/6 | 0/3 | 0/3 | 1/6 |
| M41R-nsp10.15.16rep | 0/3 | 1/3 | 2/5 | 2/3 | 0/3 | 1/5 | |
| M41R-nsp10.14.16rep | 3/3 | 1/3 | 3/6 | 3/3 | 2/3 | 0/6 | |
| M41R-nsp10.14.15rep | 3/3 | 3/3 | 2/5 | 2/3 | 3/3 | 0/5 | |
| M41R-nsp10.15rep | 0/3 | 0/3 | 0/6 | 0/3 | 0/3 | 0/6 | |
| M41-K | 2/3 | 0/3 | 6/6 | 3/3 | 0/3 | 1/6 | |
| M41-CK | 2/3 | 2/3 | 4/4 | 3/3 | 1/3 | 2/4 | |
| 3 | Mock | 0/3 | 0/3 | 0/6 | 0/3 | 0/3 | 0/6 |
| M41R-nsp10.16rep | 3/3 | 0/3 | 2/6 | 3/3 | 3/3 | 3/6 | |
| M41R-nsp10.14rep | 2/3 | 1/3 | 3/6 | 3/3 | 3/3 | 3/6 | |
| M41-K | 3/3 | 1/3 | 4/5 | 3/3 | 3/3 | 4/5 | |
| M41-CK | 3/3 | 1/3 | 1/6 | 3/3 | 3/3 | 4/6 | |
a
Virus presence was determined from randomly selected birds; results are displayed as the number of positive birds/total number of birds sampled. All positive RNA/virus isolation samples were spot-sequenced across nsps 10, 14, 15, and 16 to confirm the nucleotides at positions 12,137, 18,114, 19,047, and 20,139, respectively; no sequence changes were identified.