Table 2.
The genetic mutations between the SA113 parental isolates and its clemastine-induced tolerant T1 clone by the whole-genome sequencinga
| Ref_gene_ID | Mutate type | NA mutations | AA mutations | Subject description |
|---|---|---|---|---|
| SA113_GM000130 | Nonsyn | C742T | E248K | Teichoic acids export ABC transporter ATP-binding subunit TagH |
| SA113_GM000378 | Nonsense | C370T | Q124X | Anthranilate synthase component I |
| SA113_GM000779 | Nonsyn | G523A | A175T | Hypothetical protein |
| SA113_GM001242 | Nonsyn | C573T | A191V | Biotin synthase BioB |
| SA113_GM001742 | Nonsyn | T156C | L52S | Cyclic-di-AMP phosphodiesterase GdpP |
| SA113_GM001742 | Syn | C1019T | I339I | Cyclic-di-AMP phosphodiesterase GdpP |
| SA113_GM001857 | Nonsyn | C6513A | A2171E | Amino acid adenylation domain-containing protein |
| SA113_GM002086 | Nonsyn | C192T | R64H | Acetyl-CoA carboxylase biotin carboxylase subunit |
a
The S. aureus SA113 clone was serially subcultured in TSB containingunder clemastine pressure from 50 μM until 300 μM with 50 μM increasing concentrations every 5 days. The individual clone was isolated from the 30-day (D30) induction SA113 strain and untreated control SA113 strain, and detected by the whole-genome sequencing. NA, nucleotide; AA, amino acid.