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. 2022 Mar 23;96(6):e00002-22. doi: 10.1128/jvi.00002-22

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This article is made available via the PMC Open Access Subset for unrestricted noncommercial re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

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FIG 6 — SARS-CoV-2 nucleocapsid (N) protein disrupts the P-body formation of DDX6 and MOV10. (A) Disruption of P-body formation of endogenous DDX6 by ectopically expressed SARS-CoV-2 N. 293T cells transfected with 200 ng of pcDNA3.1-SARS-CoV-2 N (37) were stained with anti-SARS-CoV-2 nucleocapsid (GTX632269 [6H3]) and anti-DDX6 (A300-460A) antibodies. (B) Disruption of P-body formation of HA-tagged DDX6 by ectopically expressed SARS-CoV-2 N. 293T cells cotransfected with 200 ng of pcDNA3-HA-DDX6 (9) and either 200 ng of pcDNA3.1-SARS-CoV-2 N or pcDNA3 were stained with anti-SARS-CoV-2 nucleocapsid and anti-HA (3F10) antibodies. (C) Disruption of P-body formation of HA-tagged MOV10 by SARS-CoV-2 N and colocalization of SARS-CoV-2 N and HA-MOV10. 293T cells cotransfected with 200 ng of pcDNA3-HA-MOV10 and either 200 ng of pcDNA3.1-SARS-CoV-2 N or pcDNA3 were stained with anti-SARS-CoV-2 nucleocapsid and anti-HA antibodies. (D) Colocalization of endogenous DDX21 and ectopically expressed SARS-CoV-2 N in nucleoli. 293T cells transfected with 200 ng of pcDNA3.1-SARS-CoV-2 N were stained with anti-SARS-CoV-2 nucleocapsid and anti-DDX21 (A300-627A) antibodies. (E) Colocalization of HA-tagged DDX1 and SARS-CoV-2 N. 293T cells cotransfected with 200 ng of pcDNA3-HA-DDX1 (9) and either 200 ng of pcDNA3.1-SARS-CoV-2 N or pcDNA3 were stained with anti-SARS-CoV-2 nucleocapsid and anti-HA antibodies. (F) Subcellular localization of HA-tagged DDX3 and SARS-CoV-2 N. 293T cells cotransfected with 200 ng of pHA-DDX3 (8 –12) and either 200 ng of pcDNA3.1-SARS-CoV-2 N or pcDNA3 were stained with anti-SARS-CoV-2 nucleocapsid and anti-HA antibodies.