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. 2022 Mar 23;96(6):e01657-21. doi: 10.1128/jvi.01657-21

FIG 4.

FIG 4

DCLK1 promoted ALV-J replication. (A and B) Overexpression of DCLK1 promoted the transcription level of ALV-J. DF-1 cells were transfected with the control plasmid (pcDNA3.1-Mock) and pcDNA3.1-DCLK1 and then infected with ALV-J, and total RNA was extracted from cells. DCLK1 (A) and ALV-J env (B) genes were measured by qPCR. (C) Overexpression of DCLK1 promoted the expression of ALV-J protein. Total protein was extracted from the transfected cells, and then DCLK1 and ALV-J SU protein levels were detected by Western blotting. (D and E) Interference with DCLK1 reduced ALV-J mRNA level. DF-1 cells were transfected with shRNAs (shDCLK1-1, shDCLK1-2, or shNC) and then infected with ALV-J, and total RNA was extracted from the transfected cells. DCLK1 (D) and ALV-J env (E) genes were measured by qPCR. (F) Interference with DCLK1 reduced ALV-J protein level. Total protein was extracted from the transfected cells, and then DCLK1 and SU protein levels were detected by Western blotting. Data are means and SD from at least three independent experiments. *, P ≤ 0.05; **, P ≤ 0.01.