FIG 5.

DCLK1 promoted EMT in ALV-J infected cells. (A) DCLK1 promoted the mRNA expression of EMT markers in ALV-J-infected cells. Transcript levels of EMT markers were detected using qPCR after 48 h of DCLK1 overexpression in ALV-J-infected DF-1 cells. (B) DCLK1 promoted the expression of EMT marker proteins in ALV-J-infected cells. The changes in the levels of DCLK1 and EMT markers in the above-mentioned cells were detected by Western blot analysis. (C) Levels of DCLK1 and EMT marker proteins in panel B are shown as fold change after normalization to β-actin levels. Data are means and SD from at least three independent experiments. **, P ≤ 0.01.