FIG 1.

HCV infection promotes phosphorylation of HECT-type E3 ubiquitin ligase Itch via the ROS/JNK signaling pathway. (A) Huh-7.5 cells were infected with HCV J6/JFH1 at an MOI of 2. At 3, 6, and 8 days postinfection (dpi), the cells were harvested, and the cell lysates were analyzed by immunoblotting with the indicated antibodies. The level of GAPDH served as a loading control. (B) Huh-7.5 cells were infected with HCV J6/JFH1 at an MOI of 2. At 3 dpi and 6 dpi, the cells were harvested with or without pretreatment with JNK inhibitor SP600125 (SP; 30 μM for 30 h) or antioxidant N-acetyl cysteine (NAC; 5 mM for 8 h). The cell lysates were analyzed by immunoblotting with the indicated antibodies. The level of GAPDH served as a loading control.