FIG 1.

Developing a screening system by primary luciferase-based reporter screen for flagellar biosynthesis of H. pylori in multi-well plates and identification of active small compounds by the screen. A) Results of luciferase flagellar reporter screen for one library of more than 1200 compounds (LOPAC library, library 2); each dot represents one compound result. The x axis shows the number of tested compounds, the y axis shows reporter activity in percentage of a positive noninhibited control which was set to 100%. Inhibitory as well as enhancing activities of the compounds were observed. Yellow markings depict the borders of the Z factor, corresponding to the 3-fold standard error of the positive (noninhibited) controls. Dots outside the Z-factor margins are considered inhibitory (hit compounds) or enhancing effects. B) Identification of active inhibitory compounds in small compound libraries (see also Table 1) using the primary flagellar luciferase reporter screen. An upper cut-off of 70% inhibition was used to identify and enumerate the strongly inhibitory actives in the primary screen. Active compounds are given in percentage of total screened compounds in each library.