FIG 3.

CD44 overexpression promotes IBDV replication. (A–C) The IBDV VP2 expression was upregulated at 48 hpi in the overexpressed group compared with the nonoverexpressed group (A). Gray value ratio of VP2/β-actin for Fig. 2A (B). The viral load of IBDV was increased in the chCD44-overexpressed cell lines at 48 hpi than the nonoverexpressed group by RT-qPCR analysis (P < 0.05) (C). (D) 50 MOI vvIBDV was added to the CD44 overexpression cell lines and incubated for 1 h at 4°C, and the binding capability of vvIBDV to CD44-overexpressed cell lines were detected by RT-qPCR assay. IBDV copy number was increased in the overexpressed cell lines than the nonoverexpressed group by RT-qPCR analysis (P < 0.05).