FIG 1.

Phosphorylation of HPV-18 E6 upon E6AP knockdown. HeLa (A) and C41 (C) cells were subjected to siRNA-mediated knockdown of E6AP and E6/E7, with luciferase (Luci) as control. After 72 h, cells were harvested and analyzed by Western blotting, using antibodies specific to total E6, pE6, and E6AP; α-tubulin was used as a loading control. (B and D) The histogram shows the statistical significance of changes in pE6 in the absence of E6AP from at least three independent experiments. **, P value < 0.005; ns, nonsignificant; statistically quantified using Student's t test. error bars indicate the standard deviation of the mean.