FIG 2.

Knockdown of E6AP induces phosphorylation in response to p53 upregulation. (A) HeLa cells were transfected with siRNAs against E6AP, E6/E7, and p53. After 72 h, cells were harvested and analyzed by Western blotting. Proteins were probed using antibodies specific to pE6, total E6, p53, and E6AP; α-tubulin was used as a loading control. (B) The histogram shows the statistical significance of changes in pE6 levels in the absence of E6AP and in the absence of E6AP + p53 from three independent experiments with a P value of <0.005 (**), statistically quantified using Student's t test; error bars indicate the standard deviation of the mean.