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. 2022 Mar 23;96(6):e01899-21. doi: 10.1128/jvi.01899-21

FIG 1.

FIG 1

Schematic diagram depicting the deletion of multigene family (MGF) genes. (A) The genome region of African swine fever virus (ASFV) Georgia 2007/1 genotype II strain (accession number FR682468.1) is shown from MGF360-8L to MGF505-5R. The MGF360 genes are outlined in black, and MGF505 genes are outlined in purple. (B) From the same isolate, GeorgiaΔMGF was produced by the interruption of genes MGF360-9L and MGF505-4R (shaded gray) and the deletion of MGF360-10L, -11L, -12L, -13L, -14L, and MGF505-1R, -2R, and -3R. The deleted genes were replaced with the reporter gene GUS under the control of ASFV VP72 promoter (shown in blue). (C) GeorgiaΔK145RΔMGF(A) was produced from GeorgiaΔK145R by deleting MGF505-1R, MGF360-12L, MGF360-13L, and MGF360-14L and replacing these genes with a fluorescent reporter gene, mNeonGreen (shown in green) driven by the ASFV VP30 promoter. To facilitate removal of the reporter cassette by cre-recombinase, loxP sites were added at both sides of the cassette. (D) In the same manner, the recombinant GeorgiaΔK145RΔMGF(B) was produced by the deletion of MGF360-13L, MGF360-14L, MGF505-2R, and MGF505-3R genes and insertion of the mNeonGreen reporter gene under the control of the VP30 promoter. (E) The GeorgiaΔK145RΔMGF360-12LΔMGF505-1R gene-deleted virus was produced by deleting MGF505-1R and MGF360-12L and inserting the mNeonGreen gene under the control of the P30 promoter. (F, G) Single MGF gene deletion viruses GeorgiaΔK145RΔMGF505-1R (F) and GeorgiaΔK145RΔMGF360-12L (G) were produced by deleting either MGF505-1R or MGF360-12L and inserting the mNeonGreen gene under the control of the P30 promoter.