FIG 1.

Combined feature analysis. (A) A 3D scatterplot of IAV, IBV, ICV, IDV, ISAV, Thogotovirus, and TiLV proteins, based on relative ORF length, pI values, and evolutionary rates (dN/dS). Each point corresponds to a single dataset, and the colors represent different protein functions. TiLV ORFs are in black, and the numbers correspond to the protein (segment) number. A total of 431 alignments encompassing 14,025 sequences were used to generate the plot (see Materials and Methods). (B) Quantitative prediction of the linear discriminant analysis. Shown is a heatmap displaying the probability that each of TiLVs’ proteins belongs to one of orthomyxoviruses’ six known protein functions. Colors correspond to the prediction probability.