TABLE 1.
Oligodeoxynucleotides and their uses
| Oligodeoxynucleotide | Sequence (5′–3′)a | Application |
|---|---|---|
| seg4Fw pACT2homo | ATGGCCATGGAGGCCCCGGGAATGGTGAGAACTACAAAG | Cloning ORF4 in pACT2 |
| Seg4Rev pACT2homo | TCATAGATCTCTCGAGCTCGCTATCTCCCAACAGCCC | Cloning ORF4 in pACT2 |
| seg7Fw pACT2homo | ATGGCCATGGAGGCCCCGGGAATGTCCTACAAGATTGGT | Cloning ORF7 in pACT2 |
| Seg7Rev pACT2homo | TCATAGATCTCTCGAGCTCGTTAGAGTTCAAACGTGATT | Cloning ORF7 in pACT2 |
| seg8Fw pACT2homo | ATGGCCATGGAGGCCCCGGGTATGGCTCAAATCCCAACA | Cloning ORF8 in pACT2 |
| Seg8Rev pACT2homo | TCATAGATCTCTCGAGCTCGTTATTTAAGCATTTCACGG | Cloning ORF8 in pACT2 |
| gen-seg7-Fw-MS2-2-homo | AGAATTCCGGCTAGAACTAGTGGATCCCCCGGGGCAAATCTTTCTCTCATG | Cloning Segment 7 fragment in pIIIA/MS2-2 |
| gen-seg7-Rev-MS2-2-homo | ATCCTCATGTTTTCTAGAGTCGACCTGCAGGCATGCAGCTGTTTCGTTGTCTTA | Cloning Segment 7 fragment in pIIIA/MS2-2 |
| gen-seg1-Fw-MS2-2-homo | AGAATTCCGGCTAGAACTAGTGGATCCCCCGGGCCTGCCATGAGAAAGTAA | Cloning Segment 1 fragment in pIIIA/MS2-2 |
| gen-seg1-Rev-MS2-2-homo | ATCCTCATGTTTTCTAGAGTCGACCTGCAGGCATGCCAAAAGTACCACGCTCAC | Cloning Segment 1 fragment in pIIIA/MS2-2 |
| pACT2 ORF4 FW. pBluescript SK homo (w. EcoRI) | ATCGATAAGCTTGATATCGAATTCCGATGTATAAATGAAAGAAATTG | Cloning ORF4 from pACT2 in pBluescript SK+ |
| pACT2 ORF4 Rev. pBluescript SK homo (w. EcoRI) | GGATCCCCCGGGCTGCAGGAATTCTACCCCACCAAACCCAA | Cloning ORF4 from pACT2 in pBluescript SK+ |
| pACT2 upstream to ORF4 FW | TATCTACGATTCATAGATCT | Insertion detection in pACT2-ORF4 |
| pACT2 downstream to ORF4 REV | TCATATGGCCATGGA | Insertion detection in pACT2-ORF4 |
| A SAMK FW | GGCTCA GCT ATGAAGGTGATAAAAGCCTCAGGG | Site directed mutagenesis- K134A |
| B SAMK Rev | CACCTTCAT AGC TGAGCCCAGTGCAGGCACAC | Site directed mutagenesis- K134A |
| A SKMA FW | GGCTCAAAGATG GCT GTGATAAAAGCCTCAGGG | Site directed mutagenesis- K136A |
| B SKMA Rev | CAC AGC CATCTTTGAGCCCAGTGCAGGCACAC | Site directed mutagenesis- K136A |
| A SAMA Rev | GCCTTCCAGACCTCAATG | Site directed mutagenesis- K134A and K136A |
| B SAMA FW | GGCACCAGAAGCAGGGAT | Site directed mutagenesis- K134A and K136A |
| A LAVCV FW | TTA GCT GTGTGTGTTCGCATTGAGGTCTGGAAGGCTAG | Site directed mutagenesis- R158A |
| A LAVCV Rev | AATCCCCACTCAGATATGG | Site directed mutagenesis- R158A |
| B LAVCV Rev | GCGAACACACAC AGC TAATAGAGAGTTATGGTCCTTC | Site directed mutagenesis- R158A |
| B LAVCV FW | GCCTGCACTGGGCTCAA | Site directed mutagenesis- R158A |
| A ASEAAAEA FW | GAA GCTGCTGCT GAGGCTGAGAACGCTAAGAAATC | Site directed mutagenesis- K(90,91)A/R(92,94)A |
| A ASEAAAEA Rev | GTATTCAGTCCATCTGCT | Site directed mutagenesis- K(90,91)A/R(92,94)A |
| B ASEAAAEA Rev | AGCCTCAGCAGCAGCTTCACTGGCTCTCAGAAC | Site directed mutagenesis- K(90,91)A/R(92,94)A |
| B ASEAAAEA FW | TGTGATATCTGATGCTGCT | Site directed mutagenesis- K(90,91)A/R(92,94)A |
| qSeg1 Fw | TTAGCACCCAGCGGTGG | qPCR of Segment 1 |
| qSeg1 Rev | TGGTCCCTCATAGGCTC | qPCR of Segment 1 |
| qSeg2 Fw | GAGACTTGTCAGCCGTAAGTAAG | qPCR of Segment 2 |
| qSeg2 Rev | GGCAGAACTGAGGTCACAATAA | qPCR of Segment 2 |
| qSeg3 Fw | GTGTACTGTCATCCGCAATCT | qPCR of Segment 3 |
| qSeg3 Rev | GTAAGGACGAGTTCAGGAAAGG | qPCR of Segment 3 |
| qSeg4 Fw | CACTGTTGTCCTCCCTGTTAAA | qPCR of Segment 4 |
| qSeg4 Rev | GTACAAGCTGAACTCCCAAGAA | qPCR of Segment 4 |
| qSeg5 Fw | GTAGCTCTCCAATCACCTCTTC | qPCR of Segment 5 |
| qSeg5 Rev | CGTTCTGCACTGGGTTACA | qPCR of Segment 5 |
| qSeg6 Fw | ACGCTTGACGTGTAGTTTGA | qPCR of Segment 6 |
| qSeg6 Rev | ATGAGTTGGCTTAGGGTGATAAG | qPCR of Segment 6 |
| qSeg7 Fw | GTTGGGTGACTCGTCAATACA | qPCR of Segment 7 |
| qSeg7 Rev | GCCTCGAACGTATGGTTCTT | qPCR of Segment 7 |
| qSeg8 Fw | TGACCCTTGCGGTTTGTTAT | qPCR of Segment 8 |
| qSeg8 Rev | CCGAAAGCGGATAGAGGAAAG | qPCR of Segment 8 |
| qSeg9 Fw | TTCTTGGTGCCTGCCTTT | qPCR of Segment 9 |
| qSeg9 Rev | CAGGAGAGTGCAATGGTGATAG | qPCR of Segment 9 |
| qSeg10 Fw | CCCTTCTTGATCTTCCGACTTC | qPCR of Segment 10 |
| qSeg10 Rev | CAGGATGAGTGTGGCAGATTAT | qPCR of Segment 10 |
| E-11 β-actin-F | CACTGTGCCCATCTACGAG | qPCR of E-11 β-actin |
| E-11 β-actin-R | CCATCTCCTGCTCGAAGTC | qPCR of E-11 β-actin |
a
TiLV sequences are indicated in italics. Mutated nucleotides are indicated in boldface.